Journal: Scientific Reports
Article Title: Development of an objective index, neural activity score (NAS), reveals neural network ontogeny and treatment effects on microelectrode arrays
doi: 10.1038/s41598-021-88675-w
Figure Lengend Snippet: Enhancement of neural network ontogeny is easily quantified using neural activity score. ( a ) BrainPhys-based culture media results in clear enhancement of neural activity compared to traditional DMEM/Neurobasal-based media, and this enhancement is quantifiable via NAS (p < 0.0001, two-way repeated measures ANOVA, n = 24/group). ( b ) Muscle-conditioned media treatment results in similar enhancement of neural activity (p < 0.0001, two-way repeated measures ANOVA, n = 12/group).
Article Snippet: Cells were maintained with media changes every 3–4 days with full neural culture media consisting of BrainPhys Basal Media (STEMCELL Technologies, Vancouver, BC, Canada) or Advanced DMEM/F12 (ThermoFisher, Waltham, MA) and AB2 Basal Neural Media (ArunA Bio) (1:1) supplemented with 10% (v/v) KnockOut Serum Replacement (ThermoFisher), 2 mM l -glutamine (ThermoFisher), 1% penicillin/streptomycin (ThermoFisher), 0.1 mM β-mercaptoethanol (Sigma), 10 ng/mL glial-derived neurotrophic factor (GDNF) (Peprotech Inc., Rocky Hill, NJ), and 10 ng/mL ciliary neurotrophic factor (CNTF) (Peprotech).
Techniques: Activity Assay